I analyzed the Ova-specific splenocytes and lymphocytes on D14 with MHC tetramers. The regimen is a primary injection on D0 followed by a booster injection on D7. I'm performing an In vivo experiment in which I inject mice with a DC vaccine containing ovalbumin.
#Flowjo compensation matrix software
It's also a question of who will use the software in the lab - if it is only you and you are already a FlowJo expert you may stick with it, but if there are people less adept with it I think Cytof is easier to learn, and since there is less flexibility analyses from other users are easier to understand and to review.
But if you need something different, there is no way to change it. On the other hand, Flowjo also has some features which at least I have not found yet in Cytobank (overlay of scatterplots for example), and it allows for more fine tuning, customizing and parameters to be defined by the user (like setting gates for compensation manually etc.), whereas Cytobank sometimes appears a bit like an Apple product - very good, very intelligent, perfect for 90% of all situations. So, when you do Cytof or more than 15 channel FACS and you want to use tools like Spade or Visne there is probably no way around it. Cytobank has very nice and modern looking graphs, a very intuitive and intelligent work flow and user-interface and is very good with high-throughput and especially multi-channel analyses.
In my opinion it depends a lot on what you plan to do with it and what kind of "style" you prefer.
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